The aim of the present study to enhance carotenoid production of Dunaliella sp. under
stress conditions such as NaCl stress, Nitrogen, sulphur, phosphorous depletion and
supplementation of sodium bicarbonate. Pigments were analyzed using spectrophotometer every
7 days interval and amount of carotenoid influenced by these stresses were analyzed by HPLC.
Dunaliella salina produced optimum carotenoid production were seen in supplementation of
sodium bicarbonate 100mM and 150mM concentration (180.6μg/100mg and 180.5μg/100mg) in
the medium and for Dunaliella bardawil also shows optimum production of carotenoid in 50mM
concentration of sodium bicarbonate (8.34μg/100mg). The results indicated that supplementation
of sodium bicarbonate in the culture medium could be effectively manipulated to enhance
carotenoid production by D. salina and D. bardawil.